Detection of encrypted traffic generated by peer-to-peer live streaming applications using deep packet inspection

The number of applications using the peer-to-peer (P2P) networking paradigm and their popularity has substantially grown over the last decade. They evolved from the le-sharing applications to media streaming ones. Nowadays these applications commonly encrypt the communication contents or employ protocol obfuscation techniques. In this dissertation, it was conducted an investigation to identify encrypted traf c ows generated by three of the most popular P2P live streaming applications: TVUPlayer, Livestation and GoalBit. For this work, a test-bed that could simulate a near real scenario was created, and traf c was captured from a great variety of applications. The method proposed resort to Deep Packet Inspection (DPI), so we needed to analyse the payload of the packets in order to nd repeated patterns, that later were used to create a set of SNORT rules that can be used to detect key network packets generated by these applications. The method was evaluated experimentally on the test-bed created for that purpose, being shown that its accuracy is of 97% for GoalBit.A popularidade e o número de aplicações que usam o paradigma de redes par-a-par (P2P) têm crescido substancialmente na última década. Estas aplicações deixaram de serem usadas simplesmente para partilha de ficheiros e são agora usadas também para distribuir conteúdo multimédia. Hoje em dia, estas aplicações têm meios de cifrar o conteúdo da comunicação ou empregar técnicas de ofuscação directamente no protocolo. Nesta dissertação, foi realizada uma investigação para identificar fluxos de tráfego encriptados, que foram gerados por três aplicações populares de distribuição de conteúdo multimédia em redes P2P: TVUPlayer, Livestation e GoalBit. Para este trabalho, foi criada uma plataforma de testes que pretendia simular um cenário quase real, e o tráfego que foi capturado, continha uma grande variedade de aplicações. O método proposto nesta dissertação recorre à técnica de Inspecção Profunda de Pacotes (DPI), e por isso, foi necessário 21nalisar o conteúdo dos pacotes a fim de encontrar padrões que se repetissem, e que iriam mais tarde ser usados para criar um conjunto de regras SNORT para detecção de pacotes chave· na rede, gerados por estas aplicações, afim de se poder correctamente classificar os fluxos de tráfego. Após descobrir que a aplicação Livestation deixou de funcionar com P2P, apenas as duas regras criadas até esse momento foram usadas. Quanto à aplicação TVUPlayer, foram criadas várias regras a partir do tráfego gerado por ela mesma e que tiveram uma boa taxa de precisão. Várias regras foram também criadas para a aplicação GoalBit em que foram usados quatro cenários: com e sem encriptação usando a opção de transmissão tracker, e com e sem encriptação usando a opção de transmissão sem necessidade de tracker (aqui foi usado o protocolo Kademlia). O método foi avaliado experimentalmente na plataforma de testes criada para o efeito, sendo demonstrado que a precisão do conjunto de regras para a aplicação GoallBit é de 97%.Fundação para a Ciência e a Tecnologia (FCT

Genetic and microenvironmental intra-tumor heterogeneity impacts colorectal cancer evolution and metastatic development

© The Author(s) 2022 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.Colorectal cancer (CRC) is a highly diverse disease, where different genomic instability pathways shape genetic clonal diversity and tumor microenvironment. Although intra-tumor heterogeneity has been characterized in primary tumors, its origin and consequences in CRC outcome is not fully understood. Therefore, we assessed intra- and inter-tumor heterogeneity of a prospective cohort of 136 CRC samples. We demonstrate that CRC diversity is forged by asynchronous forms of molecular alterations, where mutational and chromosomal instability collectively boost CRC genetic and microenvironment intra-tumor heterogeneity. We were able to depict predictor signatures of cancer-related genes that can foresee heterogeneity levels across the different tumor consensus molecular subtypes (CMS) and primary tumor location. Finally, we show that high genetic and microenvironment heterogeneity are associated with lower metastatic potential, whereas late-emerging copy number variations favor metastasis development and polyclonal seeding. This study provides an exhaustive portrait of the interplay between genetic and microenvironment intra-tumor heterogeneity across CMS subtypes, depicting molecular events with predictive value of CRC progression and metastasis development.This work was financed by national funds from FCT - Fundação para a Ciência e a Tecnologia, I.P., in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences - UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB. This research was also funded by: PTDC/MED-ONC/28660/2017 from Fundação para a Ciência e Tecnologia (FCT) to A.R.G. A.R.G is recipient of Researcher Grant CEECIND/02699/2017 from FCT. The biobanking of CRC samples from Hospital Santa Maria, Lisbon, Portugal was supported by FCT research grant PIC/IC/82821/2007. This work was produced with the support of INCD funded by FCT and FEDER under the project 22153-01/SAICT/2016.info:eu-repo/semantics/publishedVersio

Characterisation of microbial attack on archaeological bone

As part of an EU funded project to investigate the factors influencing bone preservation in the archaeological record, more than 250 bones from 41 archaeological sites in five countries spanning four climatic regions were studied for diagenetic alteration. Sites were selected to cover a range of environmental conditions and archaeological contexts. Microscopic and physical (mercury intrusion porosimetry) analyses of these bones revealed that the majority (68%) had suffered microbial attack. Furthermore, significant differences were found between animal and human bone in both the state of preservation and the type of microbial attack present. These differences in preservation might result from differences in early taphonomy of the bones. © 2003 Elsevier Science Ltd. All rights reserved

ATLANTIC ANTS: a data set of ants in Atlantic Forests of South America

International audienc

Brazilian Flora 2020: Leveraging the power of a collaborative scientific network

International audienceThe shortage of reliable primary taxonomic data limits the description of biological taxa and the understanding of biodiversity patterns and processes, complicating biogeographical, ecological, and evolutionary studies. This deficit creates a significant taxonomic impediment to biodiversity research and conservation planning. The taxonomic impediment and the biodiversity crisis are widely recognized, highlighting the urgent need for reliable taxonomic data. Over the past decade, numerous countries worldwide have devoted considerable effort to Target 1 of the Global Strategy for Plant Conservation (GSPC), which called for the preparation of a working list of all known plant species by 2010 and an online world Flora by 2020. Brazil is a megadiverse country, home to more of the world's known plant species than any other country. Despite that, Flora Brasiliensis, concluded in 1906, was the last comprehensive treatment of the Brazilian flora. The lack of accurate estimates of the number of species of algae, fungi, and plants occurring in Brazil contributes to the prevailing taxonomic impediment and delays progress towards the GSPC targets. Over the past 12 years, a legion of taxonomists motivated to meet Target 1 of the GSPC, worked together to gather and integrate knowledge on the algal, plant, and fungal diversity of Brazil. Overall, a team of about 980 taxonomists joined efforts in a highly collaborative project that used cybertaxonomy to prepare an updated Flora of Brazil, showing the power of scientific collaboration to reach ambitious goals. This paper presents an overview of the Brazilian Flora 2020 and provides taxonomic and spatial updates on the algae, fungi, and plants found in one of the world's most biodiverse countries. We further identify collection gaps and summarize future goals that extend beyond 2020. Our results show that Brazil is home to 46,975 native species of algae, fungi, and plants, of which 19,669 are endemic to the country. The data compiled to date suggests that the Atlantic Rainforest might be the most diverse Brazilian domain for all plant groups except gymnosperms, which are most diverse in the Amazon. However, scientific knowledge of Brazilian diversity is still unequally distributed, with the Atlantic Rainforest and the Cerrado being the most intensively sampled and studied biomes in the country. In times of “scientific reductionism”, with botanical and mycological sciences suffering pervasive depreciation in recent decades, the first online Flora of Brazil 2020 significantly enhanced the quality and quantity of taxonomic data available for algae, fungi, and plants from Brazil. This project also made all the information freely available online, providing a firm foundation for future research and for the management, conservation, and sustainable use of the Brazilian funga and flora